Table 2.
Occurrence and distribution of Giardia duodenalis genotypes in WWTP effluents in Shanghai, China
| Sampling site | No. of samples | IMS-IFA | Nested PCRa | MLG analysis | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
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| No. of positive samples (%) | Mean ± SD cysts/L (range) | tpi gene | gdh gene | β-giardin gene | No. of samples | MLG types | |||||
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| No. of positive samples (%) | Genotypes (No. of positive samples) | No. of positive samples (%) | Genotypes (No. of positive samples) | No. of positive samples (%) | Genotypes (No. of positive samples) | ||||||
| WWTP1 | 16 | 16 (100) | 7.3 ± 6.7 (0.07–17.77) | 16 (100) | A2 (16), A (KJ888992) (2), A2-ESHtpi01 (1) | 14 (88) | A2 (14), B-ESHgdh01 (1) | 15 (94) | A2 (15), A3 (5) | 14 | AII-1 (14) |
| WWTP2 | 18 | 18 (100) | 6.6 ± 7.8 (0.87–26.27) | 18 (100) | A2 (18), A (KJ888992) (9), A2-ESHtpi02 (1), B-ESHtpi (1) | 17 (94) | A2 (17), B-sh01 (JX994231) (1) | 17 (94) | A2 (17), A3 (3), A2-ESHbg (1) | 17 | AII-1 (17) |
| WWTP3 | 16 | 14 (88) | 14.4 ± 14.2 (0–48.80) | 16 (100) | A2 (15), A (KJ888992) (3) | 14 (88) | A2 (14), B-ESHgdh02 (1) | 14 (88) | A2 (14), A3 (4), A5 (1) | 13 | AII-1 (13) |
a
Data from PCR and PMA-PCR combined.