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. Author manuscript; available in PMC: 2018 Jan 29.
Published in final edited form as: Cell Rep. 2018 Jan 9;22(2):456–470. doi: 10.1016/j.celrep.2017.12.044

Figure 3. Sema5A and the Small GTPase Rap1 Are Required for Proper GC Migration.

Figure 3

(A–D) Anti-Prox1 staining of coronal brain sections through the dorsal hippocampus of P1 (A and B) WT (n = 4) and (C and D) Sema5A−/− (n = 3) mouse pups.

(E) Quantification of GC distribution defects. Results are shown as mean value ± SD. **p < 0.01, unpaired two-tailed Student’s t test.

(F–I) Binding of Sema5A-Fc fusion protein to COS7 cells expressing recombinant (F) WT PlxnA2, (G) PlxnA2 RR, (H) WT PlxnA2 and WT Rap1B, and (I) PlxnA2 and constitutively active Rap1B(Q63E).

(J) Quantification of the percentile of collapsed cells of total number of Sema5A-Fc binding cells (n = 3 independent experiments).

(K–N) Anti-Prox1 staining of coronal brain sections through the dorsal hippocampus of P1 (K and L) Rap1aflox/flox/Rap1bflox/flox (n = 4) and (M and N) Rap1aflox/flox/Rap1bflox/flox;Nex-Cre (n = 5) mouse pups.

(O) Quantification of GC distribution. Results are shown as mean value ± SD. ****p < 0.0001, unpaired two-tailed Student’s t test.

Scale bars, 200 µm in (D) and (N) and 100 µm in (I).