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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Neurochem. 2017 Nov 22;143(6):736–749. doi: 10.1111/jnc.14211

Figure 10.

Figure 10

AbSL does not recognize either monomeric or aggregated Aβ25-35 peptide. SEC-purified Aβ25-35 monomers were allowed to aggregate as described in the Methods. Panel A. ThT measurements of aggregated Aβ25-35 (blue line) and ThT control (red line). Panel B. TEM image of aggregated Aβ25-35 applied to a copper-formwar grid. The scale bar is located in the lower left-hand corner (200 nm). Panel C. Indirect ELISA in immunoplates coated with increasing concentrations of monomeric (red squares) or aggregated (blue triangles) Aβ25-35 peptide or isolated Aβ42 protofibrils (green circles). AbSL (1:5000 dilution) was used as the primary antibody and the ELISA was conducted as described in the Methods.