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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Neurochem. 2017 Nov 22;143(6):736–749. doi: 10.1111/jnc.14211

Figure 4.

Figure 4

AbSL antiserum displays selectivity in dot blot assay. Nitrocellulose membranes were spotted with 2 μL Aβ42 monomers, Aβ42 protofibrils, or Aβ42 fibrils (1.4-11 ng) and analyzed by immuno-dot blot. Panel A. Aβ42 monomers and protofibrils were applied at varying concentration to nitrocellulose and treated with AbSL (1:1,000 dilution) and anti-rabbit IgG-HRP secondary antibody as outlined in the Methods. Panel B. Two separate nitrocellulose membranes were spotted with identical samples of Aβ42 monomers, protofibrils, and fibrils. The membranes were incubated with either AbSL (1:1,000 dilution) followed by anti-rabbit IgG-HRP secondary antibody or Ab9 (1:1,000) followed by anti-mouse IgG-HRP secondary antibody.