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| Criteria | score |
| GC content 30-52% | 1 |
| Up to 5 A/U in position 15-19 of S strand | 1 per A/U |
| Inverse repeat | 1 |
| A in position 19 | 1 |
| A in position 3 | 1 |
| U in position 10 | 1 |
| G/C in position 19 | 1 |
| G in position 13 | -1 |
| Relative stability of internal siRNA duplex | 1 |
| Internal stability | 1 |
