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| Duplex features and thermodynamic | Main sequence (MS) | Control positive sequence (CPS) |
| GC content | 0 | 1 |
| A/U in position 15 | 0 | 1 |
| A/U in position 16 | 0 | 0 |
| A/U in position 17 | 1 | 1 |
| A/U in position 18 | 0 | 0 |
| A/U in position 19 | 1 | 1 |
| Inverse repeat | 1 | 1 |
| A in position 19 | 1 | 1 |
| A in position 3 | 1 | 1 |
| U in position 10 | 0 | 0 |
| G/C in position 19 | 0 | 0 |
| G in position 13 | 0 | -1 |
| Relative stability of internal siRNA duplex | 1 | 0 |
| Internal stability | 0 | 1 |
