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. 2018 Jan 29;13(1):e0191482. doi: 10.1371/journal.pone.0191482

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© 2018 Koldehoff et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — A: Alloreactivity-mediated proliferation of indolamin-2,3-dioxygenase-treated HCMV-infected AML cells co-cultured with mixed lymphocyte cultures. HCMV-infected AML cells co-cultured with mixed lymphocyte cultures from healthy controls were performed without and with 1 μM and 10 μM indolamin-2,3-dioxygenase (IDO). Panel (A) shows proliferative responses determined by H3 thymidine uptake (n = 3 experiments) and proliferative responses are presented as counts per minute (CPM). B: IFN-γ secretion by indolamin-2,3-dioxygenase-treated HCMV-infected AML cells co-cultured with mixed lymphocyte cultures. Panel (B) displays IFN-γ secretion determined by ELISpot, ELISpot results are given as spots per well. One-way-ANOVA (Friedman test) indicates that the groups were significantly different (p = 0.008) in terms of IFN-γ secretion.