. 2018 Jan 29;13(1):e0192085. doi: 10.1371/journal.pone.0192085

Table 1. Sequences of primers and probes used in this study.

Purpose	Target gene	Primer or probe name	Sequence (5’→3’)	Amplicon size (base pairs)
HRSV Generic screening	M protein	RSV-Forward	`GCAAATATGGAAACATACGTGAACA`	116
		RSV-Reverse	`GCACCCATATTGTWAGTGATGCA`
		RSV-P	`(FAM)-CTTCACgAAggCTCCACATACACAgCWg-(Eclipse)`
HRSV-A subtyping	F protein	RSV-A-Forward	`ATCAGAAAAAGTTAATGTCCA`	118
		RSV-A-Reverse	`ACACCATATAGTGGTAATTGT`
		RSV-A-P	`(FAM)-TCAAATAgTTAgACAgCAAAgTTACTCT-(BHQ1)`
HRSV-B subtyping	F protein	RSV-B-Forward	`GTTTAACAAGGACTGATAGAG`	153
		RSV-B-Reverse	`TGTTACAAAGGCTGACTT`
		RSV-B-P	`(FAM)-ACTGATCCTGCATTATCACARTACCA-(BHQ1)`
HRSV-A genotyping	G protein	GPA_RSV	`GAAGTGTTCAACTTTGTACC`	487
HRSV-A genotyping	G protein	F1	`GGCAAATAACAATGGAGTTG`	487
HRSV-B genotyping	G protein	GPB_RSV	`AAGATGATTACCATTTTGAAGT`	507
HRSV-B genotyping	G protein	F1	`GGCAAATAACAATGGAGTTG`	507