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. 2018 Jan 29;13(1):e0191973. doi: 10.1371/journal.pone.0191973

Fig 3. CD73-deficiency is associated with delayed IgG3 response after Pneumovax 23 vaccination.

Fig 3

8 to 12-week-old WT and CD73 KO mice were immunized i.p. with Pneumovax 23 and sera were analyzed by ELISA for anti-PPS3 specific Abs at day 0, 7 and 28 after vaccination. On day 28, mice were challenged by i.p. injection of S. pneumoniae (105 CFU). (A) Anti-PPS3 IgM and IgG3 OD (450–570) readings are shown (means ± standard errors are shown; *: p<0.05; **: p<0.01 by Student’s T test). (B) Combined results from 2 experiments pooling 20 WT and 19 CD73 KO mice showing IgM and IgG3 mean reciprocal titers at day 7 and 28 (means ± standard errors are shown; **: p<0.01; by One-way ANOVA; brackets are shown when significantly different from day 7 and 28 for a given group). (C) Survival of infected mice was monitored (*: p<0.05 by log-rank test; survival was compared to non-vaccinated mice, unless indicated by brackets; n.s: not significant).