Figure 2.

Functional analyses of the PAX5 gene using ESCC cell lines. PAX5 siRNA treatment was performed in NUEC1 and TE3 cells with relatively strong PAX5 mRNA expression. a: PAX5 mRNA expression was examined by qRT-PCR in negative control-transfected cells (control) and PAX5 siRNA-transfected cells (PAX5 siRNA). Significant downregulation of PAX5 was confirmed in both cell lines (P < 0.050, Student's t-test, 2-tailed). b, c: Results of WST-1 and BrdU assays. PAX5-silenced cells showed significantly increased cell proliferation ability in both NUEC1 and TE3 cell lines (P < 0.050, Student's t-test, 2-tailed).