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. 2017 Sep 21;16(23):2249–2258. doi: 10.1080/15384101.2017.1373223

Figure 2.

Figure 2.

TFPI2AS1 knockdown promotes cell proliferation in A549 cells. (A) A549 cells were transfected with control siRNA or TFPI2AS1 siRNA for 48 h and then cells were harvested for examining efficiency of knockdown. (B) A549 cells transfected with control siRNA or TFPI2AS1 were trypsinized and plated into 96-well plates for MTT assay. (C) Colony formation of A549 cells 14 days after transfection with the indicated oligonucleotides. (D) After transfection with control siRNA or TFPI2AS1 siRNA for 48 h, A549 cell proliferation was measured by a Brdu incorporation assay. The proliferating cells that recently underwent DNA synthesis were labeled with Brdu (red), and total nuclei were visualized by DAPI (blue) staining. % Brdu presented as percent of Brdu incorporated cells within the siRNA-treated A549 cell population. *P < 0.05 vs NC. Data are expressed as the mean ± SD of the experiments performed in triplicate.