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TFPI2AS1 knockdown or overexpression modulates the G1/S transition in A549 cells. A549 cells were transfected with siRNA or pcDNA3.1/TFPI2AS1-full for 48 h. The population of cells at the G1, S and G2/M phase was detected by flow cytometry (A and C). The levels of CyclinD1 and CDK2 were detected by Western blotting assay (B and D). GAPDH protein was used as endogenous normalize. *P < 0.05 vs control group. Data are expressed as the mean ± SD of the experiments performed in triplicate.
