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. 2017 Dec 4;13(11):1804–1812. doi: 10.1080/15548627.2017.1356949

Figure 2.

Figure 2.

Msn2-Msn4 link the HDAC subunit Ume6 to ATG8 transcription. Indicated strains expressing pRS416 prATG8-GFP-ATG8 were grown to log phase in SCD medium without uracil and western blot analysis was performed as described in Materials and Methods. GFP-Atg8 protein expression was normalized to the Zwf1 loading control and quantification represents Atg8 protein levels relative to WT, presented as the mean ± SD of at least 3 independent experiments.