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Specific mitochondrial respiratory complexes link TORC2-Ypk1 and Msn2-Msn4 to ATG8 expression. (A-C) Indicated strains expressing pRS416 prATG8-GFP-ATG8 were grown to log phase in SCD medium without uracil. Western blot analysis was performed as described in Materials and Methods. GFP-Atg8 protein expression was normalized to Zwf1 loading control and quantification represents Atg8 protein levels relative to WT, presented as the mean ± SD of at least 3 independent experiments.
