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. 2017 Nov 9;16(23):2272–2281. doi: 10.1080/15384101.2017.1380126

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© 2017 The Author(s). Published by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — Effects of SKAP2 RNAi on spindle migration in mouse oocytes. (A) Spindle localization in mouse oocytes. In the control group, the spindles in most oocytes migrated to the cortex, whereas in the SKAP2 siRNA-injected group, they remained at the center of the cytoplasm. Green: α-tubulin; blue: chromatin. Bar = 20 μm. (B) Percentage of oocytes with spindles at the center in the SKAP2 siRNA-injected group was higher than in the control. *Significantly different (P < 0.05). (C) Cortical granule-free domain formation after SKAP2 siRNA injection in mouse oocytes. In the control group, cortical granules were absent at the cortex near the chromosomes at the MI stage. Cortical granules were distributed consistently across the entire cortex in the SKAP2 siRNA-injected group. Green: cortical granules; blue: chromatin. Bar = 20 μm.