Figure 3.
The iKAP algorithm to profile the change in kinase activity. (A) The in silico prediction of ssKSRs and the integration of protein-protein interactions between kinases and substrates were performed using our previously reported iGPS 1.0 method.27 (B) A WKSPN was modeled using the ssKSRs and the quantification information of each phosphopeptide. (C) All single-kinase networks were extracted out and each was further separated into 2 subnetworks, including the upregulated network (ratio > 1) and downregulated network (ratio < 1). (D) The Chi-square test was performed to calculate the significant changes in kinase activity.