Figure 2.

CAR3 regulates CHRN degradation in muscle cells. Gastrocnemius from control, EAMG and EAMG mice treated with AP was homogenized in lysis buffer containing 1% NP-40 and subject to SDS-PAGE and immunoblot analysis with anti-CAR3 (A) or anti-CHRN (C) antibodies. Densitometry quantification of total CAR3 (B) or CHRN (D) over ACTB/β-actin was performed using ImageJ software. (E) Total RNA was extracted from muscles, and quantitative reverse-transcribed PCR was performed using Chrna1-specific primer pairs. The results represent the mean ± SEM of 3 independent experiments (B, D, and E). *p < 0.05.