Table 1. Functional enrichment analysis of genes showing synergistic interactions with yEVenus overexpression.

At an ε = - 0.05 cutoff value (and using a p<0.05 cutoff based on bootstrapping), 184 genes showed negative interactions with yEVenus. This gene set was tested for GO Slim category enrichment. A GO category was termed as enriched significantly, if the genes annotated to a particular GO term were significantly overrepresented (Fisher's exact test, odds ratio >1, p<0.05, FDR-corrected p<0.1) in the given gene set using the complete list of screened genes as background. N indicates the number of negative interacting genes belonging to the corresponding GO Slim category. Source file is available as Supplementary file 1.

Go.id	Term	N	Odds ratio	P value	FDR corrected P value
GO:0002181	cytoplasmic translation	11	2.27	1.52E-02	1.69E-01
GO:0006325	chromatin organization	15	1.70	4.65E-02	2.93E-01
GO:0006353	DNA-templated transcription, termination	3	4.17	4.60E-02	2.93E-01
GO:0006354	DNA-templated transcription, elongation	16	8.43	2.73E-09	2.73E-07
GO:0006360	transcription from RNA polymerase I promoter	6	5.86	1.24E-03	3.10E-02
GO:0006366	transcription from RNA polymerase II promoter	29	2.29	2.01E-04	6.71E-03
GO:0006397	mRNA processing	9	2.84	7.77E-03	1.11E-01
GO:0006414	translational elongation	5	3.31	2.45E-02	2.34E-01
GO:0006457	protein folding	10	3.06	3.26E-03	5.43E-02
GO:0007005	mitochondrion organization	28	2.59	4.13E-05	2.07E-03
GO:0009408	response to heat	7	3.17	1.05E-02	1.31E-01
GO:0009451	RNA modification	7	2.53	2.91E-02	2.34E-01
GO:0016570	histone modification	8	2.33	3.05E-02	2.34E-01
GO:0032543	mitochondrial translation	12	2.96	1.78E-03	3.55E-02
GO:0048308	organelle inheritance	5	3.23	2.68E-02	2.34E-01