Figure 3.

Terminal deletions of the 2R chromosome end are present in most white colonies. (A) Map of the 2R chromosome end with telomere shown at right (triangles). Arrowheads indicate EcoRI sites, open boxes are open reading frames and solid bars are the probes used for hybridization. (B–E) Southern blots of EcoRI-digested genomic DNAs of white and blue isolates (B, W, and P indicates the blue, white, or pale colony color from which DNA was extracted) hybridized to probes, as indicated below the blots. * Shows a white ter1-Δ isolate [shown in (B) only] that is representative of isolates missing the KLLA0B14571 fragment. The identity of the telomere restriction fragments of wild-type and ter1-28C(Taq) are indicated to the right in (E). The 2R and 1R fragments are indicated by solid arrows. The faint band at ∼6 kb seen with the telomere probing of ter1-28C(Taq) is the ter1 gene.