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. 2017 Dec 12;208(2):565–578. doi: 10.1534/genetics.117.300606

Figure 7.

Figure 7

Figure 7

K14A enhances Sgo1p recruitment to chromatin in both WT and G44S strains. Strains with the indicated H3 backgrounds were synchronized with α factor, released into fresh medium, and grown for 60 min. Cells were then processed for either Sgo1p (A) or H3 (B) ChIP. ChIP DNA was analyzed using quantitative PCR and quantified as percent of IP. Four independent biological replicates were used to prepare this data. Error bars represent SD. * P < 0.05. (C) Western blot of Sgo1p expression levels as compared to G6PDH. (D) Sgo1p is eluted from chromatin at similar salt concentrations as histone H3 in all H3 backgrounds. (E) Cells with the indicated H3 backgrounds were synchronized in S phase by hydroxyurea treatment and released into fresh medium. DNA content was assessed by PI staining and flow cytometry. Ab, antibody; ChIP, chromatin immunoprecipitation; IP, immunoprecipitation; WT, wild-type.