Figure 8.

Sgo1p binds the histone H3 tail. (A) Tail peptides from Cse4p, Cnn1p, and histone H3 were C’-tagged with GST and bound to glutathione Sepharose beads. Beads were then mixed with 6His-Sgo1p and retained protein was assessed by immunoblotting. Full-length Sgo1p is displayed in the red box. (B) H3 tail peptide was either mock treated or in vitro acetylated by recombinant Gcn5p. Acetylation of histone H3 tail peptide at K9/14 was confirmed by immunoblotting with anti-acetylated K9/14 antibodies. (C) H3 tail peptides were mixed with a 6His-SUMO-Sgo1p or 6His-SUMO control and bound to glutathione Sepharose beads. Bound materials were analyzed by immunoblotting with anti-His tag antibodies. WB, western blot.