Table 1. Term enrichment for genes with differential expression in séance-RNAi ring gland samples.
| Term (n) | Number | Fold enrichment | P-value | Genes |
|---|---|---|---|---|
| Up (360) | ||||
| Ecdysone biosynthesis (8) | 3 | 15.15 | 1.79E−10 | sad phm spok |
| Oxidoreductase (275) | 19 | 2.79 | 1.77E−06 | CG32557 Cyp18a1 sad phm CG9512 CG7675 P5CDh1 Cyp4g1 CG5167 CG31937 spok Cyp6a13 Trx-2 CG3719 Drat AOX3 Cyp6a9 Cyp317a1 v(2)k05816 |
| Glutathione transferase (39) | 5 | 5.18 | 3.12E−05 | GstS1 GstE14/nobo GstT4 GstD9 GstD10 |
| Cytochrome P450 (87) | 8 | 3.72 | 5.19E−05 | Cyp18a1 sad phm Cyp4g1 spok Cyp6a13 Cyp6a9 Cyp317a1 |
| Nuclear receptor (21) | 1 | 1.92 | ns | ftz-f1 |
| Down (248) | ||||
| Ecdysone biosynthesis (8) | 1 | 7.33 | 1.83E−02 | nvd |
| Oxidoreductase (275) | 3 | 0.64 | ns | Cyp4e2 CG14946 nvd |
| Glutathione transferase (39) | 0 | 0 | ns | n/a |
| Cytochrome P450 (87) | 1 | 0.67 | ns | Cyp4e2 |
| Nuclear receptor (21) | 4 | 11.17 | 8.07E−10 | knrl Hr4 kni Eip75B |
Manually curated term enrichment analysis. For DAVID (the Database for Annotation, Visualization and Integrated Discovery)-based GO term analysis, see Tables S1 and S2 in File S2. Bold indicates genes with known roles in ecdysone homeostasis. (n) = presents how often the term is represented in the Drosophila melanogaster genome (e.g., eight ecdysone biosynthetic genes). This analysis is based on 360 significantly upregulated and 248 significantly downregulated, as detected by RNA sequencing in phm > séance-RNAi ring gland samples vs. controls. P-values based on χ2 test. ns, not significant.