Unisexual mating phenotypes of calcineurin pathway deletion mutants. A wild-type (XL280α) strain and two independent cna1∆ (CF637 and CF645), cnb1∆ (CF1095 and CF1099), cbp1∆ (CF799 and CF802), cts1∆ (CF787 and CF794), crz1∆ (CF684 and CF688), pbp1∆ (CF1123 and CF1126), tif3∆ (CF1219 and CF1220), and puf4∆ (CF1194 and CF1195) deletion mutants were inoculated onto Murashige and Skoog medium, and incubated in the dark at room temperature for 14 days. Bar represents 200 μm.