Figure 4.

Stress response phenotypes of the calcineurin pathway single and double mutants. Stress response phenotypes were tested for: (A) XL280α and cna1∆ (CF637 and CF645), cnb1∆ (CF1095 and CF1099), cbp1∆ (CF799 and CF802), cts1∆ (CF787 and CF794), and crz1∆ (CF684 and CF688) mutants; (B) XL280α and pbp1∆ (CF1123 and CF1126), tif3∆ (CF1219 and CF1220), and puf4∆ (CF1194 and CF1195) mutants; (C) XL280α, crz1∆ (CF688) and cbp1∆ (CF799) single mutants, and crz1∆ cbp1∆ double mutants (CF1025 and CF1026); (D) XL280α, crz1∆ (CF688) and cts1∆ (CF787) single mutants, and crz1∆ cts1∆ double mutants (CF998 and CF1001); (E) XL280α, crz1∆ (CF688) and puf4∆ (CF1194) single mutants, and crz1∆ puf4∆ double mutants (CF1200 and CF1203); (F) XL280α, cna1∆ (CF637), crz1∆ (CF688) and pbp1∆ (CF1123) single mutants, and cna1∆ pbp1∆ (CF1288 and CF1289) and crz1∆ pbp1∆ (CF1143 and CF1145) double mutants; and (G) XL280α, cna1∆ (CF637), crz1∆ (CF688) and tif3∆ (CF1219) single mutants, and cna1∆ tif3∆ (CF1252 and CF1256) and crz1∆ tif3∆ (CF1231 and CF1239) double mutants. Strains were grown overnight at 30°, serially diluted 10-fold, and spot inoculated onto YPD and YPD agar medium supplemented with 5 ng/ml FK506, 20 mM DTT, 0.35 M CaCl₂, 0.5% calcofluor white (CFW), and 1% Congo red (CR). Thermosensitivity of these strains was tested by comparing growth at 30, 37, and 39°.