Figure 5.

CBP1 and PBP1 synergistically regulate unisexual reproduction and stress responses in C. deneoformans. (A) Wild-type XL280α, cbp1∆ (CF799) and pbp1∆ (CF1123) single mutants, and two cbp1∆ pbp1∆ double mutants (CF1260 and CF1268) were grown on Murashige and Skoog agar medium for 14 days. Bar represents 200 μm. (B) Stress response phenotypes were tested for wild-type XL280α, cbp1∆ (CF799), pbp1∆ (CF1123), and two cbp1∆ pbp1∆ double mutants (CF1260 and CF1268). Strains were grown overnight at 30°, serially diluted 10-fold, and spot inoculated onto YPD and YPD agar medium supplemented with 5 ng/ml FK506, 20 mM DTT, 0.35 M CaCl₂, 0.5% calcofluor white (CFW), and 1% Congo red (CR). Thermosensitivity of these strains was tested at 30, 37, and 39°.