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. 2017 Oct 5;13(12):2028–2040. doi: 10.1080/15548627.2017.1379633

Figure 2.

Figure 2.

Nrbf2 overexpression reduces APP-CTFs, and intracellular and extracellular Aβ1–40 and Aβ1-42 levels. (A) After N2a mouse neuroblastoma cells stably expressing human Swedish mutant APP695 (N2S cells) were transfected with Flag-NRBF2 plasmid or vehicle plasmid, the expression of NRBF2, full-length APP (FL-APP), APP-CTFα, and APP-CTFβ were examined by western blotting; ACTB was used as an internal control. (B) Quantitative data in Fig. 2A showed that NRBF2 overexpression significantly reduced APP-CTFs levels but not FL-APP levels. (C and D) Western blotting and quantification show that Nrbf2 overexpression does not affect sAPPα, sAPPβ, and PSEN1 levels. (E) ELISA analysis results showed that Nrbf2 overexpression significantly reduces intracellular and extracellular Aβ1–40 and Aβ1-42 levels in N2S cells. (F) Overexpression of Nrbf2 in Chinese hamster ovary cells stably transfected with a plasmid encoding human APP751 bearing the V717F mutation (7PA2 cells) reduced the expression of APP-CTFs. (G) ELISA analysis results demonstrated that Nrbf2 overexpression significantly reduces intracellular and extracellular Aβ1–40 and Aβ1-42 levels in N2S cells. Quantification data are presented as the mean ± SEM, n = 3 from independent experiments. NS, not significant; *, P < 0.05 vs. the control.