Skip to main content
. 2017 Oct 31;9(2):1494–1504. doi: 10.18632/oncotarget.22217

Figure 2. Simvastatin administration increases feature of aggressiveness in prostate cancer initiation.

Figure 2

a. Schematic representation of the experimental design. 8 week-old Pten-deficient (Ptenpc-/-; pc-/-) mice were fed with simvastatin-loaded (SIM) diet or chow for four weeks, and tissues were harvested and analysed. b. Prostate lobe weights of Pten-deficient mice fed chow (pc-/- Chow, n = 18) and SIM diet (pc-/- SIM, n = 9). VP, DLP, AP refer to ventral, dorsolateral and anterior prostates respectively. c. Histopathological characterization of the prostate. (LGPIN: Low-grade prostatic intraepithelial neoplasia; HGPIN: High-grade prostatic intraepithelial neoplasia; Cancer: prostate adenocarcinoma) (Chow, n = 9; SIM, n = 6). d. Representative histological images of the prostate. Left, H&E (Haematoxylin-Eosin) and right, Ki67 staining, showing prostate intraepithelial neoplasia (PIN) in Pten-deficient mice fed with SIM or chow. e. Quantification of Ki67 positive nuclei (%), indicating proliferating cells, in pc-/- Chow (n = 5) and pc-/- SIM (n = 6). Statistical analysis: Mann-Whitney statistic test (b, e) and Chi Square test with 2 degree freedom (c). Error bars represent median with interquartile range. N.S.: Non-significant. *p < 0.05, **p < 0.01.