Skip to main content
. 2017 Aug 17;9(2):1602–1616. doi: 10.18632/oncotarget.20308

Figure 7. Inhibition of autophagy decreases the sensitivity of K7M3 osteosarcoma cells to gemcitabine (GCB)-induced cytotoxicity.

Figure 7

A. K7M3 cells were pretreated for 30 minutes with 20μM hydroxychloroquine (HCQ), 1μM GCB, or both for 48 hours. Cell viability was measured using Trypan blue exclusion assay. B Apoptosis was evaluated using Western blot analysis with an antibody specific for cleaved caspase 3. Cells were treated with GCB alone, HCQ alone, or HCQ combined with GCB for 48 hours. After treatment, cell lysates were analyzed using Western blot. β-actin was used as a loading control. C After knockdown of BECN by shRNA, whole cell lysates were analyzed for BECN expression by Western blot. D shRNA-BECN cells and K7M3 control cells were treated with 1μM GCB for 48 hours. Cell viabilitiy was measured using Trypan blue exclusion assay. Means ± standard deviation of three independent experiments are shown. *p < 0.05 compared with control.