Figure 9. Variation in phosphorylated heat shock protein 27 (HSP27) expression after treatment with gemcitabine (GCB) or 9-nitrocamptothecin (CPT) correlates to whether blocking autophagy will lead to survival or death in K7M3 osteosarcoma cells.

A. shATG5-K7M3 cells were treated with GCB or CPT and cell viability was assessed using Trypan blue exclussion assay. Mean ± standard deviation of three independent experiments are shown. *p < 0.05 compared with control. shATG5-K7M3 cells were less sensitive to GCB but more sensitive to CPT. B K7M3 cells were lysed with RIPA buffer and subjected to Western blot analysis with the antibody specific for p-HSP27. β-actin was used as a loading control. p-Hsp27 decreases in the K7M3 GCB treated cells and increases in the K7M3 9-NC treated cells.