Figure 3. TRAIL reduces AOM/DSS-induced inflammatory signaling.

(A) C57BL/6 mice were treated with 2.5% DSS and 8 mg/kg AOM (N = 8 mice per group) and administrated TRAIL (300 μg/kg) every day. Colons were obtained at day 2, 4 and 6. (B) Damaged colon tissues are stained with H&E of PBS-treated control (top) or TRAIL-treated mice (bottom) at each time points. (C) Similar residue of inflamed colon tissues are immunostained with PCNA. The numbers of positive cells as proliferating cells are significantly increased in TRAIL-treated group. (D) The apoptotic cells were detected with TUNEL assay. (E) The number of recruited macrophages in damaged tissue was measured with staining of F4/80 and compared with control group. (F and G) The expression level of pro- or anti-inflammatory cytokines was measured with qRT-PCR. The relative level of each gene was normalized with housekeeping Cyclophilin gene. Results are presented as mean ± SD from three independent experiments.