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. 2017 Dec 7;9(2):1980–1991. doi: 10.18632/oncotarget.23118

Figure 6. miR-518f-5p differentially affects migration and adhesion of prostate cell lines.

Figure 6

(A) Transfection of miR-518f-5p Significantly increased RWPE1 migration at 24h (p = 0.0001****) and 48h (p = 0.0001****) compared to NTC. (B) miR-518f-5p significantly decreased DU145 prostate cancer cell migration at 48h compared to NTC (p = 0.0004***). Results are shown as fluorescence intensity of migratory cells normalized to fluorescence intensity of RWPE1 or DU145 cells prior to commencement of the assay. (C and D). miR-518f-5p significantly increased RWPE1 cell proliferation at 48h (p = 0.0001****) and 72h (p = 0.0001****), but had no substantial effect on DU145 cell proliferation compared to NTC. Results are shown as fold change fluorescence intensity relative to 24h fluorescence. (E) miR-518f-5p significantly decreased RWPE1 cell adhesion to fibronectin (FN) (p = 0.0001****) and basement membrane extract (BME) (p = 0.0001****) compared to NTC. (F) miR-518f-5p significantly increased DU145 cell adhesion to FN (p = 0.0469*) and BME (p = 0.0140*). Results are expressed as ratio of adherence which is fluorescence intensity of adherent cells/fluorescence intensity of total cells. All experiments consisted of mock cells (cells with transfection reagent only) and NTC cells (cells transfected with a scrambled non-targeting miRNA mimic negative control); n = 3 and all y-axes are shown as arbitrary units. Graphs were analysed using ANOVA with Dunnett's multiple testing.