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. 2017 Dec 11;9(2):2002–2016. doi: 10.18632/oncotarget.23111

Figure 5. SIRT1 interacted with Akt in cells.

Figure 5

(A) and (B) FLAG-SIRT1: interaction with HA-Akt in HEK 293T cells. Flag-SIRT1 and HA-Akt were transfected individually or co-transfected into HEK 293T cells for 3 days. The cell lysates were immunoprecipitated with anti-FLAG (Figure 5A) or anti-HA (Figure 5B) antibodies. Then, the immunoprecipitates were subjected to western blot analysis. (C) and (D) SIRT1 interacted with Akt in LNCaP cells. LNCaP cells were cultured and lysed for IP with anti-SIRT1 (Figure 5C) or anti-Akt (Figure 5D) antibodies or a control IgG antibody. Then, western blot analysis was performed.