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. 2017 Dec 15;9(2):2017–2027. doi: 10.18632/oncotarget.23326

Figure 2. miR-19a downregulates TSC1/2 and activates mTORC1 in human osteoblasts.

Figure 2

Stable OB-6 cells, expressing the lentiviral miR-19a expression vector (“LV-miR-19a”, two lines, “Line1/2”), non-sense scramble control microRNA (“miRC”), or the parental control OB-6 cells (“Ctrl”), were subjected to Western blotting assay of listed proteins (A and B). Primary human osteoblasts were infected with LV-miR-19a or miRC for 48 hours, expressions of listed proteins were shown (C and D). Stable OB-6 cells with LV-miR-19a (“Line1”) (E), as well as the primary human osteoblasts with LV-miR-19a (F), were further transfected with TSC1 cDNA construct (“wt-TSC1”, 0.20 μg/mL, for 48 hours) or the empty vector (pSuper-puro-EGFP, “Vec”), total cell lysates were subjected to Western blotting assay of listed proteins. The indicated protein band was quantified (total gray), and its value was normalized to the loading control. Experiments in this figure were repeated four times, and similar results were obtained.