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. 2017 Dec 15;9(2):2017–2027. doi: 10.18632/oncotarget.23326

Figure 5. TSC1 is the primary target of miR-19a in mediating its cytoprotective activity in osteoblasts.

Figure 5

The OB-6 osteoblastic cells were infected with lentiviral scramble control shRNA (“SCR-sh”), TSC1 shRNA (“TSC1-sh”) or plus lentiviral miR-19a expression vector (“+LV-miR-19a”), cells were further selected by puromycin, expressions of listed proteins were shown (A). The above-mentioned cells were also treated with/out Dex (1 μM) for 48 hours, cell survival (B) and death (C) were tested. The indicated protein band wan quantified, and its value was normalized to the loading control (A). Data were expressed as mean ± SD (n = 5). “Ctrl” stands for parental control cells. “C” stands for untreated control group. *p < 0.05 vs. “C”.#p < 0.05 vs. Dex treatment of “SCR-sh” cells. Experiments in this figure were repeated three times, and similar results were obtained.