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. 2017 Dec 15;9(2):2017–2027. doi: 10.18632/oncotarget.23326

Figure 6. miR-19a activates Nrf2 signaling in human osteoblasts.

Figure 6

OB-6 osteoblastic cells (A and B) or primary human osteoblasts (F), with the lentiviral miR-19a expression vector (“LV-miR-19a”) or non-sense scramble control microRNA (“miRC”), were subjected to qRT-PCR assay (A and F) and Western blotting assay (B) of listed Nrf2 pathway genes; The OB-6 cells were also treated with/out Dex (1 μM) for 12 hours, relative ROS intensity was analyzed by the DCFH-DA fluorescent dye assay (E). OB-6 cells, with “LV-miR-19a” or “miRC”, were further infected with lentiviral scramble control shRNA (“SCR-sh”) or Raptor shRNA (“Raptor-sh”) for 24 hours, or plus RAD001 (200 nM) co-treatment, qRT-PCR assay was applied to test HO-1 mRNA (C) and NQO-1 mRNA (D); Data were expressed as mean ± SD (n = 5). “C” stands for untreated control group. *p < 0.05 vs. “miRC”. #p < 0.05 vs. “SCR-sh” cells (C and D). #p < 0.05 vs. Dex treatment of “miRC” cells (E). Experiments in this figure were repeated three times, and similar results were obtained.