Figure 1. Clustering of normal bone marrow, AML, and B-lymphoblastic leukemia samples by CD45/SS gating.

(A, B) Characterization of normal human and murine bone marrow samples by CD45 gating, respectively. Blue population: T and B lymphocytes (L); green population: monocytes (Mo). Note the slightly different location of murine granulocytes (G) compared with human granulocytes because of less granularity in the cytoplasm of murine granulocytes (B). (B, C) In murine bone marrow, B lymphocytes (purple population) were also present in the classic “blast gate” (cBG, CD45^dim in CD45/SS gating). These cells had lower CD19 fluorescence intensity than B cells in the classic lymphocyte gate (blue population). (D) Blasts (red population) from murine AML (mouse #1356) with expression of c-Kit and CD11b (E) in the same location in the cBG as blasts with CD34 and c-Kit expression from patient TI with AML (red population) (F, G). Monocytes from patient TI were heterogeneous and included mature and immature monocytes (CD34pos) (H). (I-L) Blasts from both murine and human B-lymphoblastic leukemia located in the cBG (red populations) (I, J: mouse #1328, K, L: patient NS). (J) Murine B-lymphoblastic leukemia expressing CD19, not CD3. (L) Expression of CD19 and CD10 on the blasts from patient NS with common-ALL. (M-P) Cytology confirmed the presence of blasts in mice and patients (D-I). (N) Note blasts, immature and mature monocytes in patient TI (F-H). M = mouse #1356 (D, E); O = mouse #1328 (I, J); P = patient NS (K, L). M and O: bone marrow cytospins; N: blood smear; P: bone marrow smear.