Figure 5. Exogenous over-expression of Bcl-2 de-sensitizes ABC294640 in C33A cells.

mRNA (A) and protein (B) expression of Bcl-2 (endogenous and exogenous) in stable C33A cells, expressing the Bcl-2 construct (two lines, “Bcl-2-L1/L2”) or empty vector (Ad-GFP, “Vector”) were shown; cells were also treated with ABC294640 (5 μmol/L) for indicate time; cell growth (MTT assay, (C) and apoptosis (Histone DNA ELISA assay, (D) were tested. Data were shown as mean (n=5) ± standard deviation (SD). ^*p<0.05 vs. “Vector” cells. Experiments in this figure were repeated three times, and similar results were obtained.