Table 1. Treatment of MCF-7 cells with 4-OH-TAM at the indicated concentrations.
| Group | Cell number/well | Medium | TAM concentration (M) | |
|---|---|---|---|---|
| A | B | |||
| 1 | 4000 cells | 8000 cells | Complete DMEM | 0 |
| 2 | 4000 cells | 8000 cells | Complete DMEM | 1×10-7 |
| 3 | 4000 cells | 8000 cells | Complete DMEM | 3.33 × 10-7 |
| 4 | 4000 cells | 8000 cells | Complete DMEM | 1×10-6 |
| 5 | 4000 cells | 8000 cells | Complete DMEM | 3.33 × 10-6 |
| 6 | 4000 cells | 8000 cells | Complete DMEM | 1×10-5 |
| 7 | 4000 cells | 8000 cells | Complete DMEM | 3.33 × 10-5 |
| 8 | 4000 cells | 8000 cells | Complete DMEM | 1×10-4 |
| 9 | 4000 cells | 8000 cells | Complete DMEM | 3.33 × 10-4 |
| 10 | 4000 cells | 8000 cells | Complete DMEM | 1×10-3 |
| 11 | 4000 cells | 8000 cells | Complete DMEM | 3.33×10-3 |
MCF-7 cell suspension containing 200 mL of 4000 cells/well and 8000 cells/well corresponding to 20% and 40% confluences per wells of the 96-well plate were seeded. The cells in each group were treated with 4-OH-TAM at the indicated concentrations for 72 h.