Table 1. Somatic mutations identified by targeted DNA sequencing of bulk tumor and gliomaspheres derived from GBM1 and SGS1.

Gene	hg19 Coord	Base Change	Amino Acid Change	Variant Allele Frequency (Gene Copy Number)
				GBM1-B Bulk	GBM1-B P4	GBM1-B P12	SGS1-A Bulk	SGS1-A P4	SGS1-A P17	SGS1-B Bulk	SGS1-B P4	SGS1-B P18	SGS1-C Bulk	SGS1-C P4	SGS1-C P17
TET2	4:106196617	C>CCC	Q1652fs	0.27 (2)
EGFR	7:55241708	G>A	G719D	0.82 (24)	0.85 (16)	0.24 (2.6)	0.12 (2.6)	0.34 (2.8)	0.42 (2.8)	0.10 (2.4)	0.26 (2.3)	0.51 (3)	0.09 (2)	0.34 (2.8)	0.41 (2.8)
EGFR	7:55249028	C>G	R776G	0.04 (24)		0.22 (2.6)
PTEN	10:89692972	T>TA	D153ins	0.45 (1)	0.72 (1)	1.00 (1)	0.23 (2)	1.00 (1)	1.00 (1)	0.21 (1.6)	1.00 (1)	1.00 (1)		1.00 (1)	1.00 (1)
PTPN11	12:112926252	G>T	G466V								0.23 (2)
CDH1	16:68835663	T>C	V85A					0.17 (2)
TP53	17:7577121	G>A	R273C										0.05 (2)
TP53	17:7578406	C>T	R175H				0.23 (2)	0.68 (2)	0.52 (2)	0.23 (2)	0.80 (2)	0.75 (2)		0.67 (2)	0.70 (2)
TP53	17:7579359	G>A	R110C				0.10 (2)	0.33 (2)	0.48 (2)	0.09 (2)	0.20 (2)	0.24 (2)		0.33 (2)	0.30 (2)