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. 2017 Dec 21;9(2):2646–2659. doi: 10.18632/oncotarget.23522

Figure 4. RUNX2 promotes ADSCs differentiation through E-cadherin.

Figure 4

(A) Representative image of ADSC morphology under the microscope after ADSCs given LV5-RUNX2 or LV3-shE-cadherin (×200). (B) Western blot analyses of protein levels of RUNX2, E-cadherin, CK18 and ZO-1 after ADSCs given LV5-RUNX2 or LV3-shE-cadherin. β-actin was used as an internal control. (C) qRT-PCR analyses of mRNA levels of E-cadherin, CK18 and ZO-1 after ADSCs given LV5-RUNX2 or LV3-shE-cadherin. (D) Immunofluorescence analysis to assess the expression of RUNX2, E-cadherin, CK18 and ZO-1 after ADSCs given LV5-E-cadherin or LV3-shE-cadherin (×100). Nuclei are stained by 4,6-diamidino-2-phenylindole. These data are expressed as the mean ± SD (*P<0.05, **P<0.01versus induced medium group; #P<0.05, ##P<0.01 versus LV5-RUNX2 group). Each experiment was repeated at least three times.