Table 2. Tissue metabolites differentiating IDC from control subjects a) Differential metabolites identified using LC-MRM/MS, b) Differential metabolites identified using GC-MS.
| S. No | Metabolite | HMDB ID | VIP | p-value | FDR | FC | AUC |
|---|---|---|---|---|---|---|---|
| a) LC-MS | |||||||
| 1 | Guanine | HMDB00132 | 1.72 | 5.16E-11 | 1.57E-09 | 6.37 | 0.97 |
| 2 | N-Acetylgalactosamine | HMDB00212 | 1.69 | 7.65E-12 | 6.96E-10 | 4.87 | 0.97 |
| 3 | Guanosine | HMDB00133 | 1.68 | 2.16E-11 | 9.81E-10 | 7.14 | 0.96 |
| 4 | UDP | HMDB00295 | 1.59 | 9.13E-07 | 5.93E-06 | 0.59 | 0.88 |
| 5 | Uracil | HMDB00300 | 1.57 | 3.45E-08 | 4.48E-07 | 2.71 | 0.91 |
| 6 | Uridine | HMDB00296 | 1.57 | 4.52E-08 | 5.14E-07 | 2.63 | 0.92 |
| 7 | Cytosine | HMDB00630 | 1.55 | 2.51E-08 | 4.48E-07 | 3.97 | 0.91 |
| 8 | Tyrosine | HMDB00158 | 1.54 | 1.25E-09 | 2.84E-08 | 8.73 | 0.96 |
| 9 | Taurine | HMDB00251 | 1.52 | 5.23E-07 | 3.66E-06 | 5.62 | 0.88 |
| 10 | Phenylalanine | HMDB00159 | 1.49 | 5.48E-08 | 5.54E-07 | 3.98 | 0.92 |
| 11 | Creatine | HMDB00064 | 1.48 | 1.26E-05 | 6.36E-05 | 4.32 | 0.85 |
| 12 | N-Acetylglucosamine | HMDB00215 | 1.48 | 4.00E-07 | 3.03E-06 | 4.82 | 0.88 |
| 13 | Glutamine | HMDB00641 | 1.48 | 7.01E-06 | 3.75E-05 | 4.60 | 0.87 |
| 14 | 7-Methylguanosine | HMDB01107 | 1.48 | 2.97E-08 | 4.48E-07 | 4.54 | 0.92 |
| 15 | Lactic acid | HMDB00190 | 1.46 | 4.58E-06 | 2.61E-05 | 4.35 | 0.85 |
| 16 | Glutamic acid | HMDB00148 | 1.46 | 1.89E-07 | 1.72E-06 | 2.39 | 0.91 |
| 17 | Cytidine | HMDB00089 | 1.44 | 3.74E-07 | 3.03E-06 | 5.16 | 0.89 |
| 18 | Riboflavin | HMDB00244 | 1.33 | 3.94E-06 | 2.39E-05 | 3.79 | 0.90 |
| 19 | Adonitol | HMDB00508 | 1.32 | 6.12E-04 | 2.23E-03 | 2.22 | 0.78 |
| 20 | Inosine | HMDB00195 | 1.32 | 3.04E-05 | 1.46E-04 | 4.95 | 0.84 |
| 21 | Maltitol | HMDB02928 | 1.31 | 1.71E-03 | 4.85E-03 | 2.12 | 0.78 |
| 22 | Histidine | HMDB00177 | 1.30 | 1.59E-04 | 6.89E-04 | 3.28 | 0.79 |
| 23 | N-Acetylglycine | HMDB00532 | 1.28 | 4.76E-04 | 1.80E-03 | 3.09 | 0.77 |
| 24 | Ascorbic acid | HMDB00044 | 1.25 | 2.13E-03 | 5.69E-03 | 3.07 | 0.76 |
| b) GC-MS | |||||||
| 25 | Phosphoric acid | HMDB02142 | 1.71 | 7.17E-08 | 7.89E-06 | 10.72 | 0.83 |
| 26 | Cis-11,14-Eicosadienoic acid | HMDB05060 | 1.69 | 9.46E-07 | 3.47E-05 | 30.41 | 0.70 |
| 27 | 11-Eicosenoic acid | HMDB34296 | 1.67 | 2.18E-06 | 5.98E-05 | 51.94 | 0.75 |
| 28 | 9-Octadecenoic acid | HMDB00207 | 1.53 | 5.53E-05 | 6.90E-04 | 7.14 | 0.74 |
| 29 | 8,11,14-Eicosatrienoic acid | HMDB02925 | 1.53 | 1.35E-05 | 2.48E-04 | 6.87 | 0.70 |
| 30 | trans-9-Octadecenoic acid | HMDB00573 | 1.51 | 4.11E-06 | 9.04E-05 | 0.28 | 0.89 |
| 31 | Pentanoic acid | HMDB00892 | 1.42 | 2.64E-05 | 4.15E-04 | 11.99 | 0.78 |
| 32 | Nonadecanoic acid | HMDB00772 | 1.41 | 1.07E-04 | 1.07E-03 | 4.83 | 0.75 |
| 33 | 11-Octadecenoic acid | HMDB03231 | 1.40 | 4.03E-04 | 2.63E-03 | 7.07 | 0.82 |
| 34 | 3,7-Cholest-5-ene | HMDB00941 | 1.34 | 3.49E-04 | 2.63E-03 | 0.48 | 0.81 |
| 35 | Hexanoic acid | HMDB00535 | 1.32 | 2.78E-04 | 2.35E-03 | 11.87 | 0.69 |
| 36 | Octadecanoic acid | HMDB00827 | 1.30 | 5.65E-05 | 6.90E-04 | 0.39 | 0.68 |
| 37 | 5,8,11,14-Eicosatetraenoic acid | HMDB01043 | 1.29 | 1.12E-03 | 5.34E-03 | 8.63 | 0.65 |
| 38 | Inositol | HMDB00211 | 1.26 | 4.30E-04 | 2.63E-03 | 0.22 | 0.84 |
| 39 | Tetradecanoic acid | HMDB00806 | 1.24 | 1.71E-04 | 1.57E-03 | 0.34 | 0.81 |
| 40 | Carbonic acid | HMDB03538 | 1.23 | 8.48E-04 | 4.44E-03 | 7.98 | 0.76 |
| 41 | Palmitic acid | HMDB00220 | 1.23 | 1.83E-03 | 6.71E-03 | 3.78 | 0.67 |
| 42 | Eicosapentaenoic acid | HMDB01999 | 1.20 | 5.92E-03 | 1.59E-02 | 4.55 | 0.70 |
HMDB ID: Metabolite ID obtained from HMDB database, VIP score: variable of importance score obtained from OPLS-DA plot (VIP>1.2), p value: p values obtained after performing t-test (p-value<0.05), FDR: value obtained after performing false discovery test, FC: fold change (FC>1.4), AUC: area under the curve value.