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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Arthritis Rheumatol. 2018 Jan 18;70(2):298–307. doi: 10.1002/art.40352

Figure 4. SS monoclonal expansions express autoreactive antibodies.

Figure 4

(A) Antibodies from mutated (full diamonds) and revertant (open diamonds) clones from SS patients were tested by ELISA for reactivity against HEp-2 cell lysate5. Dotted and green lines show ED38-positive and mnUNGdef05 λ50 low positive control6, respectively. Horizontal lines show cutoff OD405 for positive reactivity. (B) Recombinant mutated antibody from SS59 shows anti-nuclear reactivity. (C) Antibodies from mutated and revertant clones from SS patients were tested by ELISA for polyreactivity against dsDNA, insulin, and LPS antigens5. Dotted and red lines show ED38-positive and mnUNGdef05 κ69 low positive control6, respectively. (D) Ro52/SSA and rheumatoid factor (RF) reactivity was assessed by ELISA for mutated and revertant antibodies.