Figure 1.

Experimental Approach and SMC1α Expression

(A) Schematic diagram for SMC1α expression under control of the Smc1β promoter.

(B) Relative expression of Smc1α and Smc1β in FACS-sorted 4N spermatocytes analyzed by RT-PCR. SEM was calculated using three biological and three technical repeats for each genotype. Relative expression of Smc1α was normalized to Smc1β^+/+ levels. According to Bonferroni’s multiple comparison test, the mean values for Smc1α expression (Smc1β^+/+,1a: 2.365 ± 0.1826; Smc1β^−/−: 0.8375 ± 0.0584; and Smc1β^−/−,1a: 1.720 ± 0.0767) are all significantly different (p < 0.05). The mean values for Smc1β expression in all genotypes (Smc1β^+/+,1a: 1.113 ± 0.039; Smc1β^−/−: 0.0415 ± 0.00724; and Smc1β^−/−,1a: 0.03 ± 0.0057) are significantly different (p < 0.0001) except between Smc1β^−/−,1a and Smc1β^−/− (p = 0.3441).

(C) Protein levels of SMC1α, SMC1β, SMC3, and tubulin in different genotypes.

(D and E) H1t-positive spermatocyte spreads (scale bar, 5 μm; D) and cross sections (scale bar, 50 μm; E) of all genotypes.

See also Figures S1 and S7.