Figure 4.

Sex Body Formation

(A) Spermatocyte chromosome spreads of Smc1β^+/+, Smc1β^−/−, and Smc1β^−/−,1a mice, stained with anti-SYCP3 (green) for AEs/LEs and with anti-phosphorylated H2AX (γH2AX) for DNA damage.

(B) Spermatocyte chromosome spreads of Smc1β^+/+, Smc1β^−/−, and Smc1β^−/−,1a mice, stained with anti-SYCP3 (red) for AEs/LEs and with anti-phosphorylated RNA POLII (green). Asterisk indicates sex chromosomes.

(C) Graphical representation of relative expression of ZFY1 (Smc1β^−/−,1a 0.9158 ± 0.10584, Smc1β^−/−: 2.292 ± 1.212 SD; n = 3; p < 0.0001) and ZFY2 (Smc1β^−/−,1a 1.924 ± 0.904, Smc1β^−/− 3.243 ± 1.052 SD; [n = 3; p = 0.0023]). Relative expression was normalized to Smc1β^+/+ levels.

(D–K) Spermatocyte chromosome spreads of Smc1β^+/+ and Smc1β^−/−,1a mice, stained with anti-SYCP3 (red) for AEs/LEs and with anti-SYCP1 (green) (D and F–H) and with anti-phosphorylated SMC3 (E and I–K).

(D, E, F, and I) Normal sex chromosome morphology.

(G and J) No obvious sex chromosome PAR region.

(H and K) Abnormal XY axis morphology.

See also Figure S7.