Figure 5.

Centromeres

(A) Spermatocyte chromosome spreads of Smc1β^+/+, Smc1β^−/−, and Smc1β^−/−,1a mice, stained with anti-SYCP3 (red) for AEs/LEs and with anti-CENP-A (green) for centromere.

(B and C) Spermatocyte chromosome spreads of Smc1β^+/+, Smc1β^−/−, and Smc1β^−/−,1a mice, stained with anti-SYCP3 (green) for AEs/LEs, anti-CENPA (red), and anti-H1t (gray) showing H1t-positive and H1t-negative spermatocytes, respectively. AEs/LEs stained with anti-SYCP3 and centromere with anti-CENP-A. White arrows indicate abnormal centromere structure (scale bar, 5 μm).

See also Figures S5 and S7.