REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
DMEM, GlutaMAX Supplement, pyruvate	GIBCO	Cat#31966021
Dermal Cell Basal Medium	ATCC	Cat#ATCC PCS-200-030
Fetal Bovine Serum	GE Healthcare Bio-Sciences	Cat#SV30160.03
Pen/strep stock, 10,000/10,000, 100ml	Lonza	Cat#LO DE17-602E
Hydrogen Peroxide Solution	Sigma Aldrich	Cat#216763-100ML-D
Doxorubicin hydrochloride	Tebu-bio	Cat#BIA-D1202-1
25% Glutaraldehyde solution	Thermo Fisher Scientific	Cat#MERC8.20603.1000
16% Formaldehyde (w/v), Methanol-free	Thermo Fisher Scientific	Cat#28906
Diamidino-2-phenylindole (DAPI)	Sigma-Aldrich	Cat#D9542
Critical Commercial Assays
Beta-Galactosidase Staining Kit	BioVision	Cat#K802
Click-iT EdU Alexa Fluor 488 Imaging kit	Thermo Fisher Scientific	Cat#C10337
Isolate II RNA Mini Kit	Bioline	Cat#BIO-52073
High-Capacity cDNA Reverse Transcription kit	Applied Biosystems	Cat#4368813
Universal Proble Library System	Roche	Cat#04683633001
SENSIFast Probe no-ROX One-step Kit	Bioline	Cat#BIO-76001
RNAeasy mini kit	QIAGEN	Cat#74104
Truseq RNA Sample Preparation kit	Illumina	Cat#RS-122-2001 or RS-122-2002
HiSeq PE Cluster Kit v4 – cBot	Illumina	Cat#PE-401-4001
HiSeq SBS Kit v4 (50 cycles)	Illumina	Cat#FC-401-4002
Deposited Data
Self-generated RNA-seq datasets of IRIS in fibroblasts, melanocytes and keratinocytes	ArrayExpress database	ArrayExpress: E-MTAB-5403
Experimental Models: Cell Lines
Human foreskin fibroblasts HCA2	O. Pereira-Smith (University of Texas Health Science Center, San Antonio)	N/A
Human foreskin fibroblasts BJ	ATCC	Cat#CRL-2522
Mouse Embryonic Fibroblasts (MEFs)	Self-produced	N/A
Mouse primary skin microvascular endothelial cells	Cellbiologics	Cat#C57-6064
Human Epidermal Keratinocytes Neonatal Foreskin	ATCC	Cat#PCS-200-010
Human Epidermal Melanocytes Neonatal	ATCC	Cat#PCS-200-012
Oligonucleotides
Sets of primers for qPCR	IDT	Table S1
Software and Algorithms
SRA Toolkit 2.6.2	GitHub	https://github.com/ncbi/sra-tools
FastQC software v0.11.5	Babraham Institute	https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
Trimmommatic 0.36	Usadellab	http://www.usadellab.org/cms/?page=trimmomatic
STAR-2.5.1b	GitHub	https://github.com/alexdobin/STAR/releases
DESeq2	Bioconductor	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
MetaRNaseq	CRAN	https://cran.r-project.org/web/packages/metaRNASeq/index.html
Consensus Path DB-human	Max Planck Institute	http://cpdb.molgen.mpg.de/
R and associated R-packages (pheatmap, ggplot2, ggfortify, RcolorBrewer, VennDiagram)	Bioconductor, CRAN	https://cran.r-project.org/web/packages/
ImageJ	NIH – public domain	https://imagej.nih.gov/ij/download.html