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. 2018 Jan 29;8:1767. doi: 10.1038/s41598-018-20176-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DUSP5 regulation of basal ERK signaling in CRC cells. (A) Relationship between DUSP5 expression and levels of phosphorylated and total ERK1/2 in nuclear extracts of CRC cell lines. HDAC3 was used as loading control. (B) Analysis of the correlation between DUSP5 expression normalized to HDAC3 and the p-ERK2/ERK2 ratio following densitometric analysis of the data shown in (A). (C) Effect of DUSP5 knockdown in RKO cells on cytoplasmic and nuclear levels of phosphorylated and total ERK1/2, and ERK-mediated phosphorylation of the transcription factor FRA1 (FOSL1). BRAF was used as cytoplasmic marker. (D) Effect of DUSP5 knockdown on expression of the ERK-regulated genes FOSL1 and FOS in RKO cells. (E) Effect of DUSP5 knockdown on proliferation of RKO cells grown under normal (10% FCS) or low (1% FCS) serum conditions. ^*P < 0.05.