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. 2018 Jan 25;9:53. doi: 10.3389/fimmu.2018.00053

Figure 4.

Figure 4

Blockade of catabolism of branched amino acid by BCAT inhibition induces enhanced production of IL-1β. (A) Quantitative PCR analysis for BCATc and (B) immunoblot analysis for BCAT in monocytes stimulated with or without LPS (100 ng/ml) (n = 4). (C) Quantitative PCR analysis for BCATc (left; n = 5) and immunoblot analysis for BCAT in LPS-stimulated monocytes and macrophages derived from the same donor (right). (D) The amount of cytokines in culture supernatants from monocytes (left; n = 6) and macrophages (right; n = 6). Cells were stimulated with LPS for 24 h in the absence or presence of BCAT inhibitor II. Only macrophages were given additional stimulation with ATP for the last 6 h. Bar graphs show the mean ± SEM. *p < 0.05 and **p < 0.01 by two tailed paired t-test (A,C,D).