Figure 3.

Qa-1^b-restricted CD8⁺ T cell recognize conserved peptides from human and monkey. T cell clones were tested against human T2 cells and HeLa cells and monkey COS-7 cells. (A) Human TAP-negative T2 lymphoma cells were transduced with the gene for Qa-1^b. (B) Human HeLa cells were transduced with the Qa-1^b gene and the viral TAP-inhibitor BTIP (=UL49.5). (C) TAP-proficient HeLa cells were selected for low or high surface expression of Qa-1^b. (D) Monkey COS-7 cells were rendered Qa-1^b positive by gene transfer. Twofold titration of target cells are depicted, starting with 20,000 cells per well and T cell activation was measure via IFNγ production. Data are representative for at least two independent experiments with comparable results, shown as mean and SD of triplicates.