Figure 6.

Normal behavior of peripheral T cell receptor (TCR)-transgenic Ln12 T cells. Mature peripheral T cells of the TCR-transgenic mouse were examined. (A,B) Expression of activation markers on CD8⁺ T cells in spleens of C57BL/6 or Ln12 tg mice was measured by flow cytometry. Combined data from at least eight animals are depicted, shown as mean with SEM of single markers. Student’s t-test was used for statistical analyses. (C,D) In vitro stimulation of purified CD8⁺ T cells from Ln12 tg mice with Med15 or Qdm peptide. Proliferation and IFNγ release were measured. Data are representative of at least two independent experiments. (E) Naïve CFSE-labeled CD8⁺ T cells from Ln12 tg mice were i.v. transferred into C57BL/6 or TAP1^−/−. After 8 days, blood of recipient mice was analyzed for the frequency of Ln12 tg T cells. Data shown from one of the three experiments. Each line represents an individual mouse. (F,G) Ln12 tg CD8⁺ T cells were transferred to C57BL/6 mice and subsequently vaccinated with Med15 or Qdm peptide. Frequency and activation status was followed in blood of recipient mice with the use of a congenic marker. Data shown are mean with SEM of three mice and one of the two comparable experiments is shown. (G) Intracellular IFNγ detection in purified T cells was measured by flow cytometry upon brief ex vivo incubation with dendritic cells (DC) or peptides.